Thursday, October 31, 2019

Communication, Ethics, and Society Assignment Example | Topics and Well Written Essays - 250 words - 2

Communication, Ethics, and Society - Assignment Example CNN’s story seemed more about creating a trending story on their network rather than reporting the plight of stranded passengers on a stranded cruise ship.evidently, the cruise ship’s accident was not catastrophic and that is perhaps why it elicited comical re-incarnation by live comedy shows. SPJ also requires journalists to ensure that they minimize possible harm resulting from the news they cover (Baran et al, 2012). CNN helicopters and boats kept hovering above and around the stalled ship. This act was highly insensitive to the passengers who were already nervous because it made them more tense and made them panic even more. The relatives of the passengers on the cruise ship were subjected to trauma by comparison of the ship accident to Hurricane Katrina which gave an impression of mass loss of lives. Assessments of the story point towards the conclusion that it was given unnecessary full-time coverage. The magnitude of the ship mishap was overly blown out of proportion and the story created a major hype with misdirected intentions. A story of such rating, warranted a much more honest representation of the true facts while avoiding the irresponsible comparison to Hurricane Katrina. However the story can not be said to poorly represent America because it was not a representation of the lives of Americans but an isolated case of a sea cruise gone wrong. The far the story went in terms of damaging reputations was to shed bad light on the company owning the

Tuesday, October 29, 2019

The Han and Rome Essay Example for Free

The Han and Rome Essay From 206 B.C.E. 220 C.E., the Han dynasty, in China, accumulated many political control methods, as did Imperial Rome reigning from 31 B.C.E. – 476 C.E. However, there were more differences than similarities between the two empires/dynasties. Two main differences were China and Rome’s territorial expansion and their political philosophies (mandate of heaven and monarchy). A similarity between these two empires would be their centralized government adopting bureaucracy. China put their trust in the mandate of heaven, which is a Chinese political theory that gave those in power the right to rule from a divine authority. Imperial Rome relied on a monarchy system, which put Augustus Caesar (Julius Caesar’s son) into power. Another factor that differentiated Han China and Imperial Rome was their territorial expansion. Imperial Rome expanded from Italy to Spain, Gaul, Macedonia, Asia Minor, Egypt, Greece and Britannia. However, although Han China expands throughout Asia, they felt they did not need to expand much to sustain their power. Nonetheless, what allowed Han China and Imperial Rome to resemble each other, was there centralized bureaucracies. This is a government system in which all of the important decisions are made by one or a few authorities in a region. Rowland 2 Han China (206 B.C.E. 220 C.E.) and Imperial Rome (31 B.C.E. – 476 C.E.) discriminate more with their political methods, than relate to one another. Two distinctions would be their political philosophies, mandate of heaven and monarchy, and their territorial expansion concepts. One aspect that helped them relate to one another was that they shared the ideal of a centralized bureaucracy.

Saturday, October 26, 2019

Riboflavin biosynthesis in Bacillus Subtilis

Riboflavin biosynthesis in Bacillus Subtilis Motivation: Many important problems in cell biology require the dense nonlinear interactions between functional modules to be considered. The importance of computer simulation in understanding cellular processes is now widely accepted, and a variety of simulations algorithms useful for studying certain subsystems have been designed. 1 introduction Human and animal needs vitamins and minerals to help in the growth of the body and prevent disease, obtained from food or supplements. For example, an important vitamin is riboflavin and Riboflavin is water-soluble vitamins also sources of riboflavin are milk, meat, fish, fruits and vegetables. Diets that do not contain riboflavin or poor riboflavin cause serious health problems. For example, a disorder of the digestive system, heart disease and blood vessels. Riboflavin deficiency may also cause cancer. The name riboflavin comes from ribose (the sugar whose reduced form, ribitol, forms part of its structure) and flavin, the ring-moiety which imparts the yellow color to the oxidized molecule (from Latin flavus, yellow). The reduced form, which occurs in metabolism along with the oxidized form, is colorless. Riboflavin has been used in several clinical and therapeutic situations. For over 30 years, riboflavin supplements have been used as part of the phototherapy treatment of neonatal jaundice. The light used to irradiate the infants breaks down not only bilirubin, the toxin causing the jaundice, but also the naturally occurring riboflavin within the infants blood, so extra supplementation is necessary. Riboflavin is a yellow substance which was isolated in 1879. Riboflavin name comes from ribose sugar substance and also characterized by yeast an excellent dietary source. In addition, it contains two factors which are aide Flavin adenine dinucleotide (FAD) and a Flavin mononucleotide (FMN) (Daniel, 2012). 2 Background It has been pointed out by (Bretzel, et al.1999) and recently been produced commercially by riboflavin chemical manufacturing or by fermentation, where the production of riboflavin with a purity of at least 97-98% of foodstuffs and medicines. In addition, scientists biological prefer to biological and chemical manufacturing and much more than manufacturing because of the many advantages. For example, low material cost, low waste and energy requirements due to the use of renewable energy. It has been producing a large amount of riboflavin from many organisms’ example of the use of fungi, yeast and bacteria Bacillus Subtilis (Li Wu, Q, 2007). Research indicated that since 30 years the world’s consumption of riboflavin equivalent of 1.25 Ãâ€" 106 kg per year. However, the amount of riboflavin are currently more than 3.000 tons per year (Han Lim, et al.2001). Bacillus Subtilis is a gram positive, aerobic bacteria that converts glucose directly to riboflavin. Cell metabolisms are highly dependent on environmental conditions, so the metabolic state often shifts during the cultivation period. Characterizing the transience of metabolic fluxes is important for understanding how cells responded to environmental changes. (Boyle NR, Morgan JA, 2009) Fig. 1.Riboflavin synthesis reactions As the provide chart (Riboflavin synthesis reactions) shows, riboflavin bio-product of the yeast start from Guanosine triphosphate (GTP) and there are two molecules of ribulose-5-phosphate, which leads to the production of riboflavin with enzymes encoded with RIB1, RIB2, RIB4, RIB5, RIB7 and FMN1 names which helps in the production of Flavin mononucleotide also Flavin adenine dinucleotide by enzyme FAD. Moreover, ribose involved in the production of riboflavin with 3 parts of 4-dihydroxy-2-butanone-4-P and 6 parts of 7-dimethyl-8-(1-D-ribityl) lumazine. 3 methods 3.1 In-silico (computational) model In silico models have prompted considerable interest and debate because of their potential value in predicting the properties of chemical substances for regulatory purposes. The European REACH legislation promotes innovation and encourages the use of alternative methods, but in practice the use of in silico models is still very limited. (Benfenati E. and others, 2011) 3.2 Conceptual modelling – ER diagram Conceptual modeling is a way of carrying out an analysis of a system in order to identify the types of things in the system, the actual instances of those things and the relationships between those things. Entity relationship (ER) modeling is a conceptual modeling approach commonly used in Computer Science to model a domain for the development of a database. However, ER modeling provides a useful approach to starting to think about models of biological systems. 3.3 Biological network analysis and construction using Cytoscape Cytoscape platform providing a full feature set for biological network analysis. It is an open source software platform for visualizing molecular interaction networks and biological pathways and integrating these networks with annotations, gene expression profiles and other state data. Although Cytoscape was originally designed for biological research, now it is a general platform for complex network analysis and visualization. Cytoscape core distribution provides a basic set of features for data integration, analysis, and visualization. 3.4 Exploring integrated networks using String and Ondex The Ondex is a data integration platform which enables data from diverse biological data sets to be linked, integrated and visualised through graph analysis techniques. Ondex uses a rich and flexible core data structure, which has the ability to bring together information from structured databases and unstructured sources such as biological sequence data and free text. Ondex also allows users to visualize and analyze the integrated data. Ondex is free and open-source software. STRING is a database of known and predicted protein interactions. The interactions include direct (physical) and indirect (functional) associations. Together String and Ondex are very useful in case of exploring integrated networks and simulation. 3.5 Dynamic modeling using SBML SBML is neutral with respect to programming languages and software encoding; however, its oriented towards allowing models to be encoded using XML. By supporting SBML as a format for reading and writing models, different software tools (including programs for building and editing models, simulation programs, databases, and other systems) can directly communicate and store the same computable representation of those models. This removes an impediment to sharing results and permits other researchers to start with an unambiguous representation of the model, examine it carefully, propose precise corrections and extensions, and apply new techniques and approaches — in short, to do better science (Rhoads DG, Pring M, 1998) 3.6 RuleBender and BioNetGen and a rule-based language for modelling biochemical reactions Modern biological experiments generate large amounts of data describing intracellular dynamics. Rule-based languages for describing intracellular biochemistry allow for the construction and simulation of detailed models with unprecedented scope and precision. Rule-based Models can be used to suggest new hypotheses and new ideas for future experimentation. Rule-based models can be difficult to understand and time consuming to debug. RuleBender is a free tool for constructing, debugging, simulating and analyzing rule-based biological models in the BioNetGen language. 3.7 Flux Balance Analysis using OptFlux Flux balance analysis is a mathematical approach for analyzing the flow of metabolites through a metabolic network. (Jeffrey D. Orth at el, 2010). OptFlux is an open-source and modular software to support in silico metabolic engineering tasks aimed at being the reference computational application in the field. 3.8 Stochastic simulation algorithm using BioNetGen Stochastic Simulation Algorithm (GSSA) and its variants are cornerstone techniques to simulate reaction kinetics in situations where the concentration of the reactant is too low to allow deterministic techniques such as differential equations. To generate a network in advance of a simulation, which may subsequently be carried out either by numerically solving ODEs or by implementing a stochastic simulation algorithm (SSA) (Gillespie, 2007) 4 Results 4.1 Ribose and Guanosine triphosphate (GTP) The non-specific precursors of riboflavin biosynthesis which are commonly used are guanosine triphosphate and ribulose-5-phosphate. Ribose and Guanosine triphosphate (GTP) is biologically responsible for the production of riboflavin with the help of enzymes (RIB1, RIB2, RIB4, RIB5, RIB7) that catalyze reactions to produced riboflavin, with help of (FMN) to produced Flavin mononucleotide. After that, produced Flavin adenine dinucleotide (FAD). It also has the role of a source of energy or an activator of substrates in metabolic reactions, like that of ATP, but more specific. It is used as a source of energy for protein synthesis and gluconeogenesis. GTP is essential to signal transduction, in particular with G-proteins, in second-messenger mechanisms where it is converted to guanosine diphosphate (GDP) through the action of GTPases. Rib A helps stimulate the production of riboflavin and direct link between the other proteins. In addition, is responsible for the outputs of the biochemical processes. Fig. 2.Rib A Simulation Fig. 3. Metal Phosphate Simulation Metal Phosphate, which is linked to a protein. Motivating force proton transfer of phosphate is associated. Thus, the transfer of the phosphate group of phosphate, ATP to amino acids. In conclusion, it is easy to extract the pure biological industries inexpensive and riboflavin without toxic substances. Therefore, it is expected in the future production of large amounts of riboflavin and faster by yeasts and fungi away from harmful chemicals. 4.2 Dynamic modelling of Riboflavin biosynthesis in Bacillus subtilis using SBML Riboflavin synthase catalyzes the transformation of 6,7-dimethyl-8-ribityllumazine into riboflavin in the last step of the riboflavin biosynthetic pathway. Gram-negative bacteria and certain yeasts are unable to incorporate riboflavin from the environment and are therefore absolutely dependent on endogenous synthesis of the vitamin. (Markus F. et at, 204) Provided a strategy for developing and implementing genome-scale metabolic models. Simultaneous growth and riboflavin overproduction using Bacillus subtilis were investigated using Michaelis-Menten enzyme reaction model using different modern software packages for computer biochemical systems simulation. Fig. 4.Riboflavin Wild Type Simulation of Riboflavin biosynthesis in Bacillus subtilis After re-simulating Riboflavin level has been changed. The added reactions corresponds to a sharper transition of riboflavin level from the beginning of the simulated riboflavin function. Fig. 5.Riboflavin Wild Type Simulation of Riboflavin biosynthesis in Bacillus subtilis 4.3 RuleBender and BioNetGen Riboflavin simulation Rule-based modeling involves the representation of molecules as structured objects and molecular interactions as rules for transforming the attributes of these objects. Fig. 6.Simulation of Riboflavin biosynthesis in Bacillus subtilis using RuleBender The approach allows systematic incorporation of site-specific details about protein-protein interactions into a model for the dynamics of cell regulatory networks, including processes involving cell signaling, gene regulation, and metabolism. The consequences of protein-protein interactions are difficult to specify and track with a conventional modeling approach because of the large number of protein phosphoforms and protein complexes that these interactions potentially generate. Here, we demonstrate how a rule-based model is specified in the BioNetGen language (BNGL) using the RuleBender interface and demonstrate how models can be visualized, simulated, and analyze. 4.4 Riboflavin Flux Balance Analysis Provided a strategy for developing and implementing genome-scale metabolic models. Calculated Synthesis and Biomass Flux values. Simultaneous growth and riboflavin overproduction using Bacillus Subtilis were investigated using Michaelis-Menten enzyme reaction SBML model and OptFlux Software Platform for Metabolic Engineering. Fig. 7.Wild Type Simulation of Riboflavin biosynthesis in Bacillus subtilis using OptFlux Fig. 8.Riboflavin Synthesis and Biomass Flux Values 4.5 Using the SSA with BioNetGen Which Type of Model to use ODE or SSA? At low molecule numbers: use stochastic model. At high molecule numbers: both types of simulation can be used, but SSA might require more â€Å"computing power† than the ODE simulation. When the number of molecules is high SSA behaves like ODE. A chemical reaction model can be converted into a set of ordinary differential equations (ODEs) using the law of mass action. This law states that the rate of a reaction is its rate constant times the concentration of the reactants raised to the power of their stoichiometry. A better method for reasoning about genetic circuits is to utilize Gillespie’s stochastic simulation algorithm (SSA) (Gillespie, 1977). There are several variants of the SSA. This paper uses the direct method, which is shown in Algorithm 1. The SSA takes a chemical reaction network model, M, and computes a time series simulation ÃŽ ±. The SSA is essentially a Monte Carlo algorithm, which treats each reaction as a random event. For the SSA convert concentration to molecule number is using the formula: (1) Where is number of molecules, is concentration, is the Avogadro’s constant and is cell volume. Avogadro’s constant = 6.221023 Table 2. Riboflavin Enzymes convert concentration to molecule number |Enzyme| Value C Value n R5P 5e-07 5 RibA 2e-07 2 D2B4P 5e-07 5 RibH 5e-07 5 RibE 2e-07 2 Fig. 9. Simulating Riboflavin using the SSA with BioNetGen Fig. 10. Simulating Riboflavin using the SSA with BioNetGen CONCLUSION Provided a strategy for developing and implementing genome-scale metabolic models. Calculated synthesis and biomass flux values. Simultaneous growth and riboflavin overproduction using Bacillus Subtilis were investigated using Michaelis-Menten enzyme reaction SBML. An in-silico model of riboflavin biosynthesis in B. subtilis has been successfully developed. Designed the strategy for efficient producing of riboflavin in B. subtilis. The Riboflavin biosynthesis modeling has done using stochastic simulation algorithm. Presented methodology of Riboflavin biosynthesis modeling simulation using such software complexes as: RuleBender and BioNetGen, OptFlux, String and Ondex. Designed dynamic modelling of Riboflavin biosynthesis in Bacillus subtilis using SBML language. References Benfenati E., Oonella Diaza R., Cassano A., Pardoe S., Gini GM Mays C., KnaufR., Benighaus L. (2011) The acceptance of in silico models for REACH: Requirements, barriers, and perspectives, Chemistry Central Journal, Vol 5, pp 58. http://joumal.chemistry central com/content/5/1/58. Boyle NR, Morgan JA (2009) Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol 3: 4 Bretzel, W Schurter, W Ludwig B, Kupfer, E Doswald, S Pfister, M van Loon, A (1999). Commercial riboflavin production by recombinant Bacillus Subtilis: down-stream processing and comparison of the composition of riboflavin produced by fermentation or chemical synthesis. Journal of Industrial Microbiology and Biotechnology. Issue 1, pp 19-26. Chem. 58, 36-57 Feng X, Xu Y, Chen Y, Tang YJ (2012) Integrating flux balance analysis into kinetic models to decipher the dynamic metabolism of Shewanella oneidensis MR-1. PLoS Comput Biol 8: e1002376 Gillespie, D. T. (1977). Exact stochastic simulation of coupled chemical reactions. J. Phys. Chem. 81, 2340–2361. Gillespie, D. T. (2007) Stochastic simulation of chemical kinetics. Annu. Rev. Phys. Han Lim, S Soo, J Enoch, Y. (Ed.) (2001). Microbial Production of Riboflavin Using Riboflavin Overproducers, Ashbya gossypii, Bacillus Subtilis, and Candida famate: An Overview. Jeffrey D Orth, Ines Thiele, and Bernhard ØPalsson. What is flux balance analysis? Nature biotechnology, 28(3):245– 8, March 2010. Li Wu, Q Chen, T Gan, Y Chen, X Ming Zhao, X (2007). Optimization of riboflavin production by recombinant Bacillus Subtilis RH44 using statistical designs. Applied Microbiology and Biotechnology. Issue 4, pp 783-794. Markus F., Werner R., Sabine S., Boris I., Gerald Richter, Felix R., Wolfgang E., Adelbert B. (2004) â€Å"Evolution of vitamin B2 biosynthesis: structural and functional similarity between pyrimidine deaminases of eubacterial and plant origin.†, J. Biol. Chem., vol. 279, no. 35 Rhoads DG, Pring M. The simulation and analysis by digital computer of biochemical systems in terms of kinetic models. IV. Automatic derivation of enzymic rate laws. J Theor Biol. 1998 Sep;20(3):297–313. Sauer U., Hatzimanikatis V., Bailey J.E., Hochuli M., Szyperski T., Wà ¼thrich K. (1997) Metabolic fluxes in riboflavin-producing Bacillus subtilis. Nat. Biotechnol. 15, 448–452. Stolz, J (2012). Riboflavin. http://www.nutrition.tum.de/index.php?id=115L=1 (29oct. 2014). 1 [*]To whom correspondence should be addressed.

Friday, October 25, 2019

Essays --

CONTENT Bil Topic Page 1. Introduction 2 2. The Technology Application 3 3. Data Communication Concept 4 4. Technology Capabilities 5 5. Example 6 6. Advantages and Disadvantages 7 7. Conclusion 8 8. References 9 Introduction Metro E is also know as Metropolitan-area Ethernet, Ethernet MAN or metro Ethernet. It is a network that use in the metropolitan area and it is based on the Ethernet standards. This services is commonly used to connect user or network to a larger service or the internet. The user that own a business can use this system to connect they office to one and another. The user commonly use this system because it is cheaper than SONET/SDH(Synchronous Digital Hierarchy) or PDH (Plesiochronous Digital Hierarchy) that have an interface of the same bandwidth. SONET/SDH is known as a standard protocol that transfer a multiple digital bit streams over optical fibre using a laser or highly coherent light. PDH is a technology use in telecommunication network to transport a large quantity of that also use optical fibre and also microwave radio system. The Technology Application Metro E is commonly use in metropolitan city. It being use for businessman to connect their office to one and another. Many Ethernet services allow the user to network their business in ways that either more complex or impossible with the alternative services. As an example , a single Ethernet service interface can connect multiple enterprise location for their Intranet VPNs , connect business partner or suppliers via Extranet VPNs and provide a high speed Internet connection to an Internet Service Provider. When use this Ethernet subscribe, user will be able to add or change bandwidth using the other access network service within... ... commonly use for the large business. It is use to connect the business people with other branch, connect business people with their customer. It is use widely in overseas for their business. The Metro E that can transmit large data that make it being use for large company. It will transmit large data in a less amount of time. It will less the time for the company to develop and the business will have the benefits as the amount to buy it is less. Metro E is a easy application as it is wireless and can carry everywhere at anytime. It also can be use to use that just connect to each other. References http://www.webopedia.com/TERM/S/SONET.html http://metroethernetforum.org/ http://www.centurylink.com/business/data/metro-optical-ethernet.html http://www.cisco.com/c/en/us/td/docs/solutions/Enterprise/Data_Center/HA_Clusters/HClusters/HA_ME3_6.pdf

Wednesday, October 23, 2019

Back Belts

Back belt, also known as â€Å"back support belt† is a lightweight, elastic belt worn around the waist. It becomes popular due to the medical assumption that it protects the back and many workplaces acquire the use of back belts to avoid injuries among the workers. Sakol Trading and Engineering Co. , Ltd. , a Thailand-based manufacturer of medical equipment like back belts and safety slings, guarantees that the back support belt has the comfort and durability to prevent lower back and abdominal pain and injuries during lifting of heavy loads.The belt also reminds the wearer to lift properly, offers extra support to a person’s back during prolonged sitting position and repetitive bending. However, these claims are not supported by the National Institute for Occupational Safety and Health (NIOSH), the federal institute responsible for conducting research and making recommendations for the prevention of work-related injuries and illnesses. According to NIOSH, None of the s tudies they conducted provides sufficient evidence or data to support the claims that back belt reduces the load force, prevents back and abdominal injuries or stiffens the spine.Therefore, NIOSH does not recommend the use of back belts to the workers. There are several concerns that the controversial belt creates: it restricts the mobility of a person thus reducing the elasticity of the muscles and tendons; it cause strain on the cardiovascular system and; it creates a false sense of security that may make the wearer to lift excessively heavy load. It also causes muscles to be dependent on external support. Companies should not rely solely on the back belts as a safety program.They should let their workers undergo trainings about proper lifting and back support. The workers can be allowed to use back belts if they want to since wearing of it is voluntary. However, they should remember that there is no scientific evidence to support that back belts really work. Reference: †¢ At kinson, Williams. Back Belts: Useful or Not? LP/Gas, Vol. 65, Issue 3, p. 46-47 (March, 2005). Retrieved May 28, 2009 from http://web. ebscohost. com/ehost/pdf? vid=5&hid=4&sid=d76b81c5-3c37-451b-9278-484eeeb125cb%40sessionmgr2#db=bth&AN=16409961

Tuesday, October 22, 2019

Escher essays

Escher essays Maurits Cornelis Escher was born on June 17, 1898, in Leeuwarden, Netherlands. His parents were George Arnold Escher and Sarah Gleichman Escher. They had three sons and Maurits, called Mauk for short, was the youngest. The family lived in a big and nice house named "Princessehof," which would later become a museum and hosted exhibitions of M.C. Escher's works. Young M.C. Escher and his family later moved to Arnhem. He attended elementary and secondary school there, and also in the seaside town of Zandvoort, where he lived for a while to improve his health. In 1907, he started learning carpentry and piano. In secondary school, his marks were poor except in drawing. His art teacher took an interest in his artistic talent, and taught him to make linocuts. He failed his final exam and never graduated. Also, in 1917, the Escher family moved to Oosterbeek, Holland. During this year and the following few years, M.C. Escher became very involved in literature, and began to write some of his own poems and essays. Trying to pursue a career in architecture, M.C. Escher next moved to Haarlem and began studies as the School for Architecture and Decorative Arts. There he met the artist Jessurun de Mesquita. After seeing Escher's drawings, Mesquita and the school's director advised him to continue with them. He began full-time study of the graphic and decorative arts in 1919. In 1922, Escher and a couple of friends visited Italy. He did a great deal of serious drawing in San Gimignano, Volterra, and Siena. He spent all of the spring of 1922 roaming the Italian countryside, drawing landscapes, plants, and even insects. In On June 12, 1924, Escher married Jetta in Viareggio, Italy. The new couple traveled much that year. They visited Genoa, Annecy, and Brussels. Upon their return home, Escher and Jetter bought a house just outside Rome and moved into it in October of 1925. Shortly after Escher and ...